高温环境下日粮中添加锌、维生素E和β-胡萝卜素对公牛精液品质和抗氧化指标的影响

doi:10.11843/j.issn.0366-6964.2014.04.012

Abstract

Abstract:

The effects of dietary vitamins (vitamin E and beta-carotene) and zinc on semen quality，antioxidant indicators of Holstein bull in hot weather were analyzed.Sixty healthy and purebred Holstein bulls (BW=1 010± 80 kg) were randomly classified into 4 groups (n=15)：group Ⅰ：control group (basal diet); group II：basal diet +100 mg•kg^-1DMZn; group III：basal diet+antioxidant (300 mg•kg^-1DMvitamin E and 60 mg•kg^-1DMbeta-carotene) and group IV：basal diet +100 mg•kg^-1DMZn + vitamins (300 mg•kg^-1DMvitamin E and 60 mg•kg^-1DMbeta-carotene).The trial duration was 120 days.The results showed that vitamins and zinc could significantly increase the semen quality，vitality of fresh semen (group Ⅳ 0.649 vs groupⅠ0.575)，sperm density (group Ⅳ 15.16×10⁸ mL^-1 vs groupⅠ11.81×10⁸ mL^-1)，vitality of frozen semen (group Ⅳ 0.34 vs groupⅠ0.283) and acrosome integrity rate (group Ⅳ 45.2% vs groupⅠ41.8%) but decreased the sperm deformity rate significantly (group Ⅳ13.47% vs groupⅠ 16.81%).However，there was no significant effect on ejaculate volume (group Ⅳ11.56 mL vs groupⅠ10.33 mL).In addition，the serum Cu-Zn SOD activity could be significantly increased by the supplemental vitamins or vitamins + zinc（P＜0.01）; the individual or combination of supplemental vitamins and zinc significantly positively affected the serum T-AOC（P＜0.01）; and GSH-PX activity significantly （P＜0.01）; the serum MDA (P=0.053 5) concentration and hydroxyl radicals (P=0.069 8) were markedly reduced as the result of adding vitamins and zinc.Supplemental vitamins significantly increased the total SOD（P＜0.01）and T-AOC（P＜0.01）activities in seminal plasma; the Cu-Zn SOD activity （P＜0.05） in seminal plasma was increased by the addition of zinc.In conclusion，a diet with 300 mg•kg^-1DMof vitamin E，60 mg•kg^-1DMof beta-carotenoids and 100 mg•kg^-1DMZn could improve the semen quality in hot weather.

CLC Number:

LI Qiu-feng，LI Jian-guo，WU Yun-hai，MA Ya-bin，LIU Xing-hua，GAO Yan-xia，CAO Yu-feng，LIU Ting-yu. The Effects of Vitamin E，Beta-carotene and Zinc on Semen Quality，Antioxidant Indicators in Hot Weather[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(4): 587-595.

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The Effects of Vitamin E，Beta-carotene and Zinc on Semen Quality，Antioxidant Indicators in Hot Weather

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